Dotted line: Ct value of 26.7 or 1E6 SARS-CoV-2 RNA copy numbers/mL. Horizontal bars represent median and standard deviation. Ct values, viral load and Ag-RDT results for 166 RT-PCR-positive individuals. SARS-CoV-2 detection by Panbio ™ antigen rapid test using OPS compared to the reference RT-qPCR detection method using NPS.Ī. Here we describe a prospective study comparing the diagnostic performances of an Ag-RDT using OPS with RT-qPCR using NPS for detection of SARS-CoV-2.įig 1. OPS sampling could be a useful alternative to NPS sampling, as seen with RT-qPCR tests. Currently, only validations of Ag-RDTs performed with NPS have shown satisfactory results, and no studies have evaluated Ag-RDTs using oropharyngeal swabs (OPS). The World Health Organization (WHO) considers a sensitivity ≥80% and a specificity ≥97% as acceptable performance for SARS-CoV-2 Ag-RDTs. Their drawbacks are mainly reduced sensitivity relative to RT-qPCR. Antigen rapid diagnostic tests (Ag-RDTs) are easy to use, more affordable, decentralizable, and provide quick results offering an attractive alternative to RT-qPCR during pandemics. While displaying excellent sensitivity and specificity, RT-qPCR is costly, subject to reagent and material shortages during pandemics, and requires experienced personnel and complex infrastructure. Currently, SARS-CoV-2 RT-qPCR performed on nasopharyngeal swabs (NPS) is the gold-standard diagnostic test. Large scale testing allows for identification and isolation of infected individuals, and quarantining contacts, thus limiting community transmission. The SARS-CoV-2 pandemic has killed millions of people worldwide. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. Marie Thérèse Ngo Nsoga is a beneficiary of the excellence grant from the Swiss Confederation and the grant from the humanitarian commission of the University Hospital of Geneva. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript.įunding: This work was supported by Foundation of Innovative Diagnostics (FIND), by Private HUG Foundation and by Pictet Charitable Foundation. Received: FebruAccepted: JPublished: June 24, 2021Ĭopyright: © 2021 Ngo Nsoga et al. PLoS ONE 16(6):Įditor: Nei-yuan Hsiao, University of Cape Town Faculty of Health Sciences, SOUTH AFRICA (2021) Diagnostic accuracy of Panbio rapid antigen tests on oropharyngeal swabs for detection of SARS-CoV-2. Citation: Ngo Nsoga MT, Kronig I, Perez Rodriguez FJ, Sattonnet-Roche P, Da Silva D, Helbling J, et al.
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